Sanger Sequencing & Fragment Analysis
High-accuracy DNA analysis for research and clinical applications
CRID offers trusted Sanger sequencing and fragment analysis services using advanced capillary electrophoresis technology. Our experienced team ensures fast turnaround and reliable, publication-ready results.
1. Sanger Sequencing
Gold-standard sequencing for targeted applications
Sanger sequencing remains the benchmark for:
- Mutation validation
- Cloning confirmation
- Targeted sequencing of specific genes or regions
We support a wide range of sample types, including animal, plant, insect, environmental, food, fungal, bacterial, viral, and parasitic DNA.
Our platform delivers:
- Read lengths up to 1000 bp
- Accuracy >99.99%
- Fast turnaround (within 7 working days)
Platform: ABI 3500xL Genetic Analyzer (24-capillary system)
Applications include:
- Mutation detection and verification
- Plasmid or construct confirmation
- PCR product sequencing
- CRISPR screening validation
- SNP genotyping
2. Fragment Analysis
High-resolution genotyping and fragment sizing
Our fragment analysis service supports:
- Microsatellite (SSR) genotyping
- STR profiling (e.g., forensics, cell line authentication)
- Loss of heterozygosity (LOH) analysis
- SNP detection via SNaPshot
- Mutation detection (e.g., fragment length polymorphism)
- CRISPR screening (indel analysis)
Using the ABI 3500xL, we offer:
- Accurate sizing from 50 bp to >1000 bp
- Multiplexing capabilities
- Optional expert data interpretation and troubleshooting
